Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F Pruski leaves

Mardina, Vivi and Ilyas, Syafruddin and Halimatussakdiah,, Halimatussakdiah and Harmawan, Tisna and Tanjung, Masitta and Yusof, Faridah (2021) Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F Pruski leaves. J Adv Pharm Technol Res., 12 (3). pp. 222-226. E-ISSN 3434-5598

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Abstract

This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial - using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity - using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 μg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 μg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Antibacterial, antioxidant, cytotoxicity, MCF-7, Wedelia trilobata
Subjects:	Q Science > Q Science (General) T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	12 Aug 2021 08:25
Last Modified:	12 Aug 2021 08:25
URI:	http://irep.iium.edu.my/id/eprint/91438

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