Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line

Kajahmohideen, Nur Haseena and Razi, Siti NurSyafiqah and Faisal, Ghasak Ghazi and Ashour, Abdelkader Elbadawy and Kusumawardani, Anisa and Makky, Essam A. and Ibrahim, O. E. (2021) Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line. Pharmacognosy Journal, 13 (4). pp. 1014-1018. ISSN 0975-3575

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Abstract

Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties against nasopharyngeal carcinoma (NPC), a type of cancer that shows poor prognosis for metastatic disease. The purpose of this study was to determine whether the E. longifolia root extract exerts cytotoxic activity against nasopharyngeal carcinoma (ORL-115) cell lines. Materials and Method: E. longifolia root extracts were obtained through Soxhlet extraction method and by using two different solvents; ethanol and dicholoromethane. MTS assay was used to evaluate the cytotoxic effect of the root extracts against ORL-115 cell line for three different incubation time which were 24-hour, 48-hour and 72-hour. Results: Ethanol extract was significantly more potent compared to DCM extract. Ethanol extract exhibited lower IC50 value compared to DCM extract. The IC50 of ethanol extract were 232.1 μg/ml, 66.86 μg/ml and 42.6 μg/ml. Meanwhile the IC50 of DCM extract were 678.87 μg/ml, 136.71 μg/ml, 73.72 μg/ml for 24-hour, 48-hour and 72-hour incubation period respectively. The cytotoxic activity of both extracts increased as the incubation time prolonged. The cytotoxic activity of ethanol extract at each incubation time was significantly different from DCM extract except at 72 hours. Conclusion: E. longifolia root extracts exerted cytotoxic activity against the nasopharyngeal carcinoma (ORL-115) cell line. Ethanol extract exhibited lower IC50 value compared to DCM extract. The cytotoxic activity of both extracts were dose dependent and time dependent. Key words: E. longifolia, Cytotoxic activity, Nasopharyngeal carcinoma.

Item Type:	Article (Journal)
Additional Information:	6388/90816
Uncontrolled Keywords:	E. longifolia, Cytotoxic activity, Nasopharyngeal carcinoma.
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry > Department of Restorative Dentistry Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Assoc.Prof Ghasak Ghazi Al-Atrakchi
Date Deposited:	14 Jul 2021 15:58
Last Modified:	05 Oct 2021 10:34
URI:	http://irep.iium.edu.my/id/eprint/90816

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