Ab Aziz Al Safi, Nur Amalin and Yusof, Faridah and Azmi, Azlin Suhaida (2020) Involvements of food grade dialdehydic pectin as cross-linker and soy protein as additive in the production of MNP-CLEA-lipase from Hevea brasiliensis. Journal of Applied Biotechnology and Bioengineering, 7 (5). pp. 215-223. E-ISSN 2572-8466
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Abstract
Skim latex from Hevea brasiliensis (rubber tree) contains many useful enzymes that can be utilized to produce value-added products. Lipase recovered from skim latex serum was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by magnetic nanoparticles termed ‘MNP-CLEA-lipase’. One of the main advantages of this method is, the biocatalyst can be easily recovered by a magnetic field for recycling. In this research, dialdehydic pectin was used as the cross-linking agent, replacing glutaraldehyde, and soy protein was used as an additive, replacing bovine serum albumin (BSA). The operating conditions for the preparation of the most active MNP-CLEA-lipase were optimized statistically. Dialdehydic pectin and soy protein showed optimum operating conditions at 180 mg/mL and 0.6% (w/v) respectively and with (NH4)2SO4 at 80% saturation, giving a Residual Activity (RA) of 154%. The optimum temperature for MNP-CLEA-lipase was at 40°C (127% RA) while the optimum pH was at 8 (127% RA). It retained 20% RA after 100 days of storage. The reusability test showed that it maintained 7% RA after the 10th cycle. This research showed two advantages; first, that value-added products can be recovered from agricultural waste, and second, by alternating to low-cost food-grade cross-linkers and additives, enhanced biocatalyst can be produced which can be safely used in the beverage and food industries.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 4534/83801 |
| Uncontrolled Keywords: | MNP-CLEA-lipase, Hevea brasiliensis, cross-linked enzyme aggregates (CLEA), macromolecular cross-linker, glutaraldehyde, pectin, soy protein |
| Subjects: | T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering |
| Depositing User: | DR FARIDAH YUSOF |
| Date Deposited: | 28 Oct 2020 15:38 |
| Last Modified: | 28 Oct 2020 15:39 |
| URI: | http://irep.iium.edu.my/id/eprint/83801 |
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