Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system

Elgharbawy, Amal A.M. and Alam, Md. Zahangir (2018) Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur.

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Abstract

Utilization of the abundant raw materials in Malaysia as well as surmounting the obstacle of the shortcoming of fossil fuels resources are current concerns of the research. Cellulase development aims to enhance the bioethanol production from lignocellulosic biomass such as empty fruit bunches (EFB) that resists enzymatic hydrolysis due to its complex structure. Hence, ionic liquids (ILs) have been introduced with the ability to disrupt the lignocellulose to be accessible by enzymes and thus it is expected to achieve a significant improvement by conducting both treatment and hydrolysis in one step by leaping cellulose regeneration. Palm kernel cake (PKC) was utilized as the primary media for cellulase production through solid-state bioconversion (SSB) by Trichoderma reesei. Factors that affect the hydrolysis in the ionic liquid-cellulase (IL-E) system was investigated using one-factor at a time (OFAT) and reducing sugar obtained as the response to the study. It was found that the biomass could be loaded up to 35% (w/v) and agitation has influenced the rate of reducing sugar production where increasing the speed was associated with higher concentration. Higher IL concentration in the combined system caused lower enzymatic activity and hence, less sugar was produced. The results obtained can be employed for the one-step process in the conversion of EFB into fermentable sugar.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	4157/67239
Uncontrolled Keywords:	cellulase; lignocellulose; stability; ionic liquid; pretreatment
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr AMAL ELGHARBAWY
Date Deposited:	16 Nov 2018 12:47
Last Modified:	16 Nov 2018 12:47
URI:	http://irep.iium.edu.my/id/eprint/67239

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