Isolation of thermotolerant bacteria producing fibrinolytic enzyme

Ab. Shukor , Nurul Aqilah and Amid, Azura and Abdul Karem, Mohd Ismail and Ab. Samad, Nadiah Syuhada (2015) Isolation of thermotolerant bacteria producing fibrinolytic enzyme. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Pahang. (Unpublished)

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Abstract

Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme.. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53 for 24 hours was utilized to isolated thermo tolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are mortile and cocci in shapes, with 4 types of arrangement which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus lincheniformis with 98 % similarity to Bacillus lincheniformis DCM 13 and Bacillus lincheniformis strain ATCC 14580. Therefore, HSP23 is named as B. lincheniformis starin HSP23.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	3688/44442
Uncontrolled Keywords:	fibrinolytic enzyme, thermotolerant bacteria, bacteria identification
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	28 Aug 2015 09:27
Last Modified:	01 Jan 2016 14:47
URI:	http://irep.iium.edu.my/id/eprint/44442

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