Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract

Hussah Abdullah, Al-Shwyeh and Sabo Muhammed, Abdulkarim and Abdullah, Rasadee and Mirghani, Mohamed Elwathig Saeed and Al-Qubaisi, Mothanna (2014) Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract. BMC Complementary and Alternative Medicine, 14 (199). pp. 1-15. ISSN 1472-6882 (In Press)

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Abstract

Waterlily Mango (Mangifera indica L.) is thought to be antioxidant-rich, conferred by its functional hytochemicals. Methods: The potential anticancer effects of the ethanolic kernel extract on breast cancer cells (MDAMB-231 and MCF-7) using MTT, anti-proliferation, neutral red (NR) uptake and lactate dehydrogenase (LDH) release assays were evaluated. Cytological studies on the breast cancer cells were also conducted, and phytochemical analyses of the extract were carried out to determine the likely bioactive compounds responsible for such effects. Results: Results showed the extract induced cytotoxicity in MDA-MB-231 cells and MCF-7 cells with IC50 values of 30 and 15 μg/mL, respectively. The extract showed significant toxicity towards both cell lines, with low toxicity to normal breast cells (MCF-10A). The cytotoxic effects on the cells were further confirmed by the NR uptake, antiproliferative and LDH release assays. Bioactive analyses revealed that many bioactives were present in the extract although butylated hydroxytoluene, a potent antioxidant, was the most abundant with 44.65%. Conclusions: M. indica extract appears to be more cytoxic to both estrogen positive and negative breast cancer cell lines than to normal breast cells. Synergistic effects of its ntioxidant bioactives could have contributed to the cytotoxic effects of the extract. The extract of M. indica, therefore, has potential anticancer activity against breast cancer cells. This potential is worth studying further, and could have implications on future studies and eventually management of human breast cancers.

Item Type:	Article (Journal)
Additional Information:	4971/37324
Uncontrolled Keywords:	Mangifera indica L, kernel extract, MCF-7 cells, MDA-MB-231 cell, cytotoxicity, anticancer activity
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	A Prof Elwathig Mirghani
Date Deposited:	23 Jul 2014 08:48
Last Modified:	19 Sep 2017 15:22
URI:	http://irep.iium.edu.my/id/eprint/37324

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