Amid, Azura and Jamal, Parveen and Mohamad, Nurul Elyani and Engku Abdullah, Engku Hasmah
(2011)
Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds.
In:
Current research and development in biotechnology engineering at IIUM.
IIUM Press, Kuala Lumpur, pp. 17-23.
ISBN 9789674181444
Abstract
Kenaf (Hibiscus cannabinus) is composed of various active components including
tannins, polyphenolics, alkaloids, essential oils and steroids. In this study, kenaf seeds oil
was used as a material in observing the anticancer activity on MCF-7 cancer cell line and
finding the optimum parameter to extract the oil that give the highest anticancer activity.
Kenaf variety Khon kaen seeds were extracted using Supercritical Fluid Extractor (SFE).
The extraction was done at the different level of temperature and pressure which range
from 40 to 80°C and 200 to 600 bars respectively. Among these two parameters,
temperature shows the highest impact on the percentage viability ofMCF-7 breast cancer
cell line. All the oils were cytotoxic towards MCF-7 breast cancer cell line in dose
dependent manner as detected using the SRB assay. Kenaf seeds oil extracted from SFE
at temperature of 40°C and pressure of 400 bars was the most cytotoxic towards MCF-7
breast cancer cell line with the IC50 value of 321.43 ug/ml.
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