Metal chelating activity of Centella asiatica

Mohd Salim, Roshan Jahn and Amid, Azura and Adenan, Mohd Ilham and Sued, Anee Suryani (2010) Metal chelating activity of Centella asiatica. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang.

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Abstract

Centella asiatica (CA), a potent antioxidant, was studied for its potential as a neuroprotective agent to fight against oxidative damage by reactive oxygen species (ROS) to neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer's and Parkinson disease. The build-up of an iron gradient in conjunction with ROS is thought to constitute a major trigger in neuronal toxicity and demise in these diseases. The limited number of neuroprotective study carried out so far indicate the iron chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. The metal chelating potency can be determined by assessing the ability of neuroprotective agents to compete with ferrozine for Fe(II) ions. Ferrozine forms complexes with Fe(II) producing a red color that can be measured quantitatively at 562 nm and addition of iron chelators interferes with the formation of ferrous and ferrozine complexes in a concentration-dependent manner. The IC50 value, concentration re quired for 50% inhibition of the iron-ferrozine complex, was measured. Methanolic and aqueous extract of CA was prepared by conventional soxhlet extraction (40-60*C, ratio: 1:30(w/v), 15 hours). The IC50 of methanolic and aqueous extract obtained was 0.26mg/ml and 0.69mg/ml respectively. The (-)-epigallocatechin-3-gallate (AGCG), Major component of green tea reported to have induce neuroprotection and neurorescue both in vitro and in vivo was used as positive control. Further, Response Surface Methodology was used to determine the optimum CA extraction condition that give optimum metal chelating activity. Experiments were designed according to Central Composite Design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. Run 17 (25*C, ratio: 1:45(w/v), 1.5 hours at 200 rpm) gave the optimum chelating activity with IC50 0.093 mg/ml.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3688/19491
Uncontrolled Keywords:	Centella asiatica
Subjects:	R Medicine > R Medicine (General) R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	18 Jun 2013 12:07
Last Modified:	18 Jun 2013 12:07
URI:	http://irep.iium.edu.my/id/eprint/19491

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