Denitrification of nitrogenous compounds in industrial wastewater using Bacillus tequilensis

Jimat, Dzun Noraini and Mohd Isa, Mohamed Irfan and Engliman, Nurul Sakinah and Sulaiman, Sarina and Kamarudzaman, Ain Nihla and Abu Samah, Rozimi (2026) Denitrification of nitrogenous compounds in industrial wastewater using Bacillus tequilensis. Chemical and Natural Resources Engineering Journal, 10 (1). pp. 35-41. E-ISSN 2637-0719

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Abstract

An ideal wastewater treatment process should be simple, cost-effective, and capable of achieving high removal efficiency. In recent years, heterotrophic nitrification and aerobic denitrification (HN-AD) have gained attention as a promising approach for removing nitrogenous compounds such as ammonium nitrogen (NH₄⁺-N), nitrite nitrogen (NO₂⁻-N), and nitrate nitrogen (NO₃⁻-N). In this study, the potential of Bacillus tequilensis to perform HN-AD was evaluated using 1% v/v diluted wastewater with an initial ammonium nitrogen (NH₄⁺-N) concentration of 65 mg/L. Batch experiments were conducted with varying glucose concentrations (0–4% w/v) and incubated overnight at 37°C. The treatment performance was monitored by measuring changes in ammonium, nitrite, nitrate, pH, and bacterial growth. Without glucose supplementation, the culture showed minimal ammonium removal and considerable nitrite accumulation, suggesting incomplete nitrogen conversion. However, when glucose was added at 3% and 4% (w/v), ammonium removal improved significantly (approximately 65–72%) and nitrite accumulation gradually decreased. While nitrate levels remained relatively stable at lower glucose concentrations, a noticeable decrease was observed at the highest glucose concentration. This finding suggests that the increased carbon availability may influence the nitrogen transformation pathway and enhance overall removal performance. This result indicates that carbon availability significantly impacts nitrogen transformation by Bacillus tequilensis in high-ammonium wastewater environments.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Bacillus tequilensis, high-ammonium, ammonium-N, nitrite and nitrate
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD172 Environmental pollution
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Dzun Noraini Jimat
Date Deposited:	17 Jul 2026 09:37
Last Update:	17 Jul 2026 09:37
Queue Number:	2026-07-Q4066
URI:	http://irep.iium.edu.my/id/eprint/129934

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