Evaluation of the effect of jasmonic acid elicitation on composition of pigments and biological activities in green callus of neem (azadirachta indica)

Ahmad Fauzi, Nurul Syazwani and Abd Rahim, Muhamad Hafiz and Abdul Majid, Nazia and Othman, Rashidi and Yaacob, Jamilah Syafawati (2022) Evaluation of the effect of jasmonic acid elicitation on composition of pigments and biological activities in green callus of neem (azadirachta indica). Frontiers in Sustainable Food Systems, 6 (1017398). pp. 1-18. E-ISSN 2571-581X

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Abstract

This study was carried out with the aim of determining the effects of jasmonic acid (JA) elicitation on the bioactive pigments’ biosynthesis and the antioxidant activities in green callus of Azadirachta indica of two different ages (4- and 8-week-old). Plant tissue culture technique was employed to induce the formation of green callus from leaf explants of A. indica on Murashige and Skoog (MS) medium supplemented with 0.6 mg/L thidiazuron (CM) and three different concentrations of JA (2, 4, and 6 mg/L). The methanolic extracts from the green callus were used for determination of total chlorophyll content (TCh), total carotenoid content (TC), total anthocyanin content (TAC), total phenolic content (TPC), and total flavonoid content (TFC) through colorimetric and HPLC analyses. The highest amount of yield was obtained from CM and 2 mg/L JA (2JA) extracts for 4- and 8-week-old samples, respectively. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, and terpenoids in all 4- and 8-week-old samples elicited with 2, 4 and 6 mg/L JA. The highest value for TAC, TCh, TC, TPC, and TFC of 4- and 8-week-old samples were from callus cultured on media supplemented with 6 mg/L JA (6JA) and 4 mg/L JA (4JA), respectively. The lowest IC50 values were found to be 8.29 ± 0.10 mg/mL (6JA) for 4-week-old and 7.73 ± 0.03 mg/mL (4JA) for 8-week-old samples. The highest Ferric Reducing Antioxidant Power (FRAP) values obtained in this study were 90.60 ± 1.55 g/g (6JA), and 74.59 ± 3.91 g/g (4JA), respectively, for 4- and 8-week-old samples. Moreover, Pearson’s correlation analysis revealed a significant correlation between TAC, TCh, TC, TPC, and TFC with ABTS and FRAP assays. In addition, PCA analysis revealed that 83.5% of the information (variances) contained in the data were retained by the first two principal components. Overall, these findings suggested that JA supplementation into the culture media significantly increase the chlorophyll, carotenoid, anthocyanin, phenolic and flavonoid contents and JA concentrations at 6mg/L JA and 4mg/L JA yielded the highest pigments content in 4- and 8-weeks-old callus, respectively.

Item Type:	Article (Journal)
Uncontrolled Keywords:	jasmonic acid, Azadirachta indica, plant tissue culture, callus age, bioactive pigments, antioxidant activities
Subjects:	R Medicine > RS Pharmacy and materia medica S Agriculture > SB Plant culture T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP934 Paints, pigments, varnishes, etc
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture Kulliyyah of Architecture and Environmental Design
Depositing User:	Dr rashidi othman
Date Deposited:	02 Dec 2022 12:11
Last Modified:	02 Dec 2022 12:14
URI:	http://irep.iium.edu.my/id/eprint/101546

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