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Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds

Amid, Azura and Jamal, Parveen and Mohamad, Nurul Elyani and Engku Abdullah, Engku Hasmah (2011) Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 17-23. ISBN 9789674181444

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Abstract

Kenaf (Hibiscus cannabinus) is composed of various active components including tannins, polyphenolics, alkaloids, essential oils and steroids. In this study, kenaf seeds oil was used as a material in observing the anticancer activity on MCF-7 cancer cell line and finding the optimum parameter to extract the oil that give the highest anticancer activity. Kenaf variety Khon kaen seeds were extracted using Supercritical Fluid Extractor (SFE). The extraction was done at the different level of temperature and pressure which range from 40 to 80°C and 200 to 600 bars respectively. Among these two parameters, temperature shows the highest impact on the percentage viability ofMCF-7 breast cancer cell line. All the oils were cytotoxic towards MCF-7 breast cancer cell line in dose dependent manner as detected using the SRB assay. Kenaf seeds oil extracted from SFE at temperature of 40°C and pressure of 400 bars was the most cytotoxic towards MCF-7 breast cancer cell line with the IC50 value of 321.43 ug/ml.

Item Type: Book Chapter
Additional Information: 3688/20912
Uncontrolled Keywords: anticancer, Kenaf seeds oil, MCF-7 cell line, Supercritical Fluid Extractor (SFE)
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 03 Jul 2012 14:30
Last Modified: 03 Jul 2012 14:38
URI: http://irep.iium.edu.my/id/eprint/20912

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