A multivariate analysis of antibacterial activity of crude Carica papaya seed extract and its effectiveness as natural preservative in Asian yellow noodle

Abdullah Sani, Muhamad Shirwan and Mohamad Asri, Nurul Aimi Amanina and Othman, Rashidi and Nordin, Noor Faizul Hadry and Mohd Desa, Mohd Nasir and Azid, Azman (2026) A multivariate analysis of antibacterial activity of crude Carica papaya seed extract and its effectiveness as natural preservative in Asian yellow noodle. In: Beyond Halal: Exploring Dietary Preferences, Novel Ingredients and Techniques. Academic Press, Elsevier, pp. 221-241. ISBN 978-0-443-30058-5

PDF (Book Chapter) - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

The antibacterial activities of Carica papaya seed extract at MIC (5.63 mg/mL) was evaluated in a carbohydrate, protein and oil model systems against Salmonella enteritidis, Bacillus cereus, Vibrio vulnificus, and Proteus mirabilis to find the suitable food system. The efficacy of the extract was verified in Asian yellow noodles and studied by Principal Component Analysis (PCA), Cluster Analysis (CA), and Discriminant Analysis (DA) during storage at 30°C for 12 days. Extract at MIC inhibited the indicator microorganisms in up to 30% of starch, 15% palm oil, and 5% protein. PCA ranked variables contributing to the yellow noodle spoilage as storage day > Aw > pH > extract concentration. Storage day had a strong correlation with TPC (R = 0.842), pH (R = −0.794) and Aw (R = −0.803), respectively. Factor loading of PC2 indicated that 2MIC and 4MIC extracts rendered antibacterial activity in the noodle. The CA classified three spoilage patterns (SP): Cluster 1 (C1, low spoilage patterns) at 0–2 d; Cluster 2 (C2, moderate SP) at 2–8 d; and Cluster 3 (C3, high SP) at 10–12 d of storage, respectively. DA identified 8 days of storage could be rendered with CP seed extract. The 22.5 mg/g extract extended the shelf-life of the treated noodle as compared to 3 days (control).

Item Type:	Book Chapter
Additional Information:	7834/127626
Subjects:	H Social Sciences > HD Industries. Land use. Labor > HD9016.5 Halal Science T Technology > TP Chemical technology T Technology > TP Chemical technology > TP372.6 Halal food industry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Architecture and Environmental Design Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture
Depositing User:	Ts. Dr AMAL ELGHARBAWY
Date Deposited:	09 Mar 2026 12:20
Last Modified:	09 Mar 2026 16:06
Queue Number:	2026-03-Q2461
URI:	http://irep.iium.edu.my/id/eprint/127626

Actions (login required)

View Item