Rismayuddin, Nurul Alia Risma and Mohd Badri, Puteri Elysa Alia and Ismail, Ahmad Faisal and Othman, Noratikah and Bandara, H.M.H.N and Arzmi, Mohd Hafiz (2022) Synbiotic Musa acuminata skin extract and Streptococcus salivarius K12 inhibit candida species biofilm formation. Biofouling, 38 (6). pp. 614-627. ISSN 0892-7014 E-ISSN 1029-2454 (In Press)
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Abstract
This study aimed to determine the effect of synbiotic Musa acuminata skin extract (MASE) and Streptococcus salivarius K12 (K12) on Candida species biofilm formation. Liquid chromatography quadrupole time-of-flight (LC-Q-TOF-MS) was conducted to characterize MASE. To determine the effect of synbiotic on Candida biofilm, 200 mL of RPMI-1640 containing Candida, K12, and MASE were pipetted into the same well and incubated at 37�C for 72h. A similar protocol was repeated with K12 or MASE to determine the probiotic and prebiotic effects, respectively. Dimorphism, biofilm biomass, and Candida total cell count (TCC) were determined. A total of 60 compounds were detected in MASE. C. albicans (ALT5) and Candida lusitaniae exhibited the highest reduction in biofilm biomass when co-cultured with prebiotic (77.70 ± 7.67%) and synbi- otic (97.73 ± 0.28%), respectively. All Candida spp. had decreased TCC and hyphae when co-cul- tured with synbiotic. In conclusion, MASE and K12 inhibit Candida biofilm formation.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Musa acuminata; Streptococcus salivarius; Candida species; symbiotic; prebiotic; biofilm |
| Subjects: | Q Science > QR Microbiology R Medicine > RK Dentistry |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences |
| Depositing User: | AP Ts Dr Mohd Hafiz Arzmi |
| Date Deposited: | 28 Aug 2022 16:07 |
| Last Modified: | 28 Aug 2022 16:07 |
| URI: | http://irep.iium.edu.my/id/eprint/99673 |
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