Effects of flaxseed (linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth

Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina (2021) Effects of flaxseed (linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth. Makara Journal of Health Research, 25 (1). pp. 1-7. ISSN 2356-3664 E-ISSN 2356-3656

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Abstract

Background: Flaxseed promotes bone health and possibly function in inducing bone regeneration process. Although flaxseed extract affects bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. This study aimed to determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4 mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR (RT-PCR) for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposition in SHED. Results: All osteoblast markers were expressed in all samples analysed. OSX expression appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED.

Item Type:	Article (Journal)
Additional Information:	7172/89811
Uncontrolled Keywords:	Flaxseed extract, human exfoliated deciduous teeth, osteoblast differentiation, stem cells
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	12 May 2021 11:23
Last Modified:	12 May 2021 11:23
URI:	http://irep.iium.edu.my/id/eprint/89811

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