Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion

Abdul Karim, Mohamed Ismail (2009) Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion. In: Seminar on Research Findings Series No. 2, Kuala Lumpur, Malaysia. (Unpublished)

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Abstract

A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4684/8909
Uncontrolled Keywords:	BRIN-BD11, D-glucose, L-glutamine, Insulin
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Mohamed Ismail Abdul Karim
Date Deposited:	04 Apr 2012 11:41
Last Modified:	04 Apr 2012 14:54
URI:	http://irep.iium.edu.my/id/eprint/8909

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