Ternary glycerol-based deep eutectic solvents: physicochemical properties and enzymatic activity

Rashid, Shahidah Nusailah and Hayyan, Adeeb and Hayyan, Maan and Hashim, Mohd Ali and Elgharbawy, Amal Ahmad and Sani, Fazrizatul Shakilla and Basirun, Wan Jefrey and Lee, Vannajan Sanghiran and Alias, Yatimah and Mohammed, Alaa Kareem and Mirghani, Mohamed Elwathig Saeed and Zulkifli, M.Y. and Rageh, Maher (2021) Ternary glycerol-based deep eutectic solvents: physicochemical properties and enzymatic activity. Chemical Engineering Research and Design, 169 (5). pp. 77-85. ISSN 0263-8762

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Abstract

The present study investigates deep eutectic solvents (DESs) as potential media for enzymatic hydrolysis. A series of ternary ammonium and phosphonium-based DESs were prepared at different molar ratios by mixing with aqueous glycerol (85%). The physicochemical properties including surface tension, conductivity, density, and viscosity were measured at a temperature range of 298.15 K – 363.15 K. The eutectic points were highly influenced by the variation of temperature. The eutectic point of the choline chloride: glycerol: water (ratio of 1: 2.55: 2.28) and methyl triphenylphosphonium bromide:glycerol: water (ratio of 1: 4.25:3.75) is 213.4 K and 255.8 K, respectively. The stability of the lipase enzyme isolated from the porcine pancreas (PPL) and Rhizopus niveus (RNL) toward hydrolysis in ternary DESs medium was investigated. The PPL showed higher activity compared to the RNL in DESs. Molecular docking simulation of the selected DES with the substrate (p-nitrophenyl palmitate) towardPPL was also reported. It is worth noting that ternary DES systems would be viable lipaseactivators in hydrolysis reactions.

Item Type:	Article (Journal)
Additional Information:	4971/88992
Uncontrolled Keywords:	Pancreatic lipase, Green solvent, Rhizopus niveus lipaseIonic liquid, Choline chloride, Molecular docking simulation, Enzymatic hydrolysis,
Subjects:	Q Science > Q Science (General) T Technology > T Technology (General) T Technology > T Technology (General) > T55.4 Industrial engineering.Management engineering.
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering
Depositing User:	A Prof Elwathig Mirghani
Date Deposited:	24 Mar 2021 09:27
Last Modified:	04 May 2021 14:14
URI:	http://irep.iium.edu.my/id/eprint/88992

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