Biomass enhancement of Stevia Rebaudiana Bertoni shoot culture in temporary immersion system (TIS) RITA® bioreactor optimized in two different immersion periods

Melviana, Agustine Christela and Esyanti, Rizkita Rachmi and Mel, Maizirwan and Setyobud, Roy Hendroko (2021) Biomass enhancement of Stevia Rebaudiana Bertoni shoot culture in temporary immersion system (TIS) RITA® bioreactor optimized in two different immersion periods. In: The 1st International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICoN BEAT 2019), 7th-8th November 2019, Malang, Indonesia.

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Abstract

Stevia plant contains steviol glycosides, which are estimated to be 300 times sweeter than sucrose. However, conventional (in vivo) propagation of Stevia rebaudiana in Indonesia was not effective due to poor results. Therefore, an alternative method to propagate stevia plants is needed. One of them is by using in vitro method. Multiplication with a large quantity of stevia biomass in a relatively short period can be conducted by using TIS RITA® (Recipient for Automated Temporary Immersion System). This study aimed to evaluate the effect of the immersion period of the medium on growth and the medium bioconversion into the production of shoot biomass. The bioreactors were set up with 15 min and 30 min immersions periods, scheduled every 6 h, and incubated for 21 d. The result indicated that the immersion period affected the biomass and growth rate (μ). Amount of 30 min immersion showed a greater percentage of shoot multiplication, higher biomass, percentage of leaf growth, growth rate, and productivity compared to 15 min immersion. The pattern of sucrose, mineral, and inorganic compounds consumption followed the growth of plant biomass for both systems. In conclusion, 30 min immersion gave a greater efficiency medium bioconversion to plant biomass compared with 15 min immersion.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4085/87652
Uncontrolled Keywords:	Diabetes prevention, micropropagation, natural sweetener, sweetener plant, tissue culture
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	12 Jan 2021 14:23
Last Modified:	07 May 2021 15:16
URI:	http://irep.iium.edu.my/id/eprint/87652

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