rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh

Salam, Md. Abdus and Mondal, Dinesh and Kabir, Mamun and Haque, Rashidul (2009) rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh. Pakistan Journal of Mededical Sciences, 25 (4). pp. 635-640. ISSN 1682-024X E-ISSN 1681-715X

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Abstract

Objective: The diagnostic role of rK39 enzyme linked immunosorbent assay (ELISA) was compared with the buffy-coat Leishmania nested polymerase chain reaction (Ln-PCR) for kala-azar in an endemic zone of Bangladesh. Methodology: This evaluation study included 100 cases comprising of 60 clinically suspected patients of kala-azar and 40 healthy controls. Anti-rK39 antibody was estimated in plasma by ELISA and buffy coat was utilized to detect Leishmania DNA by Ln-PCR from both patients and controls. The diagnostic role of rK39 ELISA was then compared with Ln-PCR positive cases which were considered as confirmed cases of kala-azar. Results: Out of 60 suspected patients of kala-azar, 58 (96.67%) were found positive in ELISA against 56 (93.33%) of Ln-PCR-positive cases. Further, 55 (98.21%) of ELISA-positive patients were also found positive in Ln-PCR. Voluntary healthy controls (40) included 20 persons from the endemic zone and 20 from non-endemic zone of kala-azar, were all found negative in both Ln-PCR and rK39-ELISA. The sensitivity and specificity of ELISA were found to be 98.21% and 95.65% respectively. Conclusion: rK39-ELISA is a non-invasive serodiagnostic tool for visceral leishmaniasis with very high sensitivity and specificity.

Item Type:	Article (Journal)
Additional Information:	9217/87231
Uncontrolled Keywords:	Visceral leishmaniasis, Kala-azar, rK39-ELISA, Ln-PCR, Sensitivity, Specificity.
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Prof. Dr. Md. Abdus Salam
Date Deposited:	07 Jan 2021 10:51
Last Modified:	07 Jan 2021 10:51
URI:	http://irep.iium.edu.my/id/eprint/87231

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