Flaxseed (Linum usitatissimum) extract activity on human oral fibroblasts (HOrF) cell line

Naziyah Shaaban, Sama and Mokhtar@Makhtar, Khairani Idah and Arief Ichwan, Solachuddin Jauhari and Ashour, Abdelkader Elbadawy Abbas and Mustafa Al-Ahmad, Basma Ezzat (2020) Flaxseed (Linum usitatissimum) extract activity on human oral fibroblasts (HOrF) cell line. Annals of Dentistry University of Malaya, 27 (8). pp. 50-54. ISSN 0128-7532 E-ISSN 2462-2060

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Abstract

Natural products have demonstrated various activities beneficial to general health. Flaxseed (Linum usitatissimum) has been reported in many studies for its antimicrobial, antioxidant, and anti-inflammatory effects. Additionally, flaxseed extracts have skin wound healing activity and potential for treating oral ulcers. L. usitatissimum was extracted using 70% ethanol via soxhlet method and gas chromatography mass spectrum (GC-MS) was used to analyze the components of L. usitatissimum extract. The crude flaxseed oil were applied to human oral fibroblasts (HOrF), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability after 24, 48 and 72 hours. Scratched HOrF cells were treated with crude flaxseed oil and healing was monitored per wound healing assay. GC-MS indicate that the major components present in L. usitatissimum oil extract are linolic, palmitic and oleic acid. L. usitatissimum crude oil extract showed high proliferation effect on HOrF cells at 24 and 48 hours, while the highest proliferation effect was recorded at 72 hours post-treatment. The wound healing assay results showed that healing activity of HOrF cells occurred as soon as 18 hours post-treatment when treated with L. usitatissimum crude oil extract. L. usitatissimum crude oil extract has proliferating and healing effects on HOrF cell line. Therefore, it can be considered as a potential promising oral wound healing agent.

Item Type:	Article (Journal)
Additional Information:	7172/85348
Uncontrolled Keywords:	L. usitatissimum, GC-MS, MTT, HOrF, wound healing assay
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Medicine
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	02 Dec 2020 00:16
Last Modified:	02 Dec 2020 00:16
URI:	http://irep.iium.edu.my/id/eprint/85348

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