Study of cells attachment using impedance spectroscopy technique

Nordin, Anis Nurashikin and S.M., Arifuzzaman and Mel, Maizirwan and Spray, David and Voiculescu, Ioana (2011) Study of cells attachment using impedance spectroscopy technique. In: International Mechanical Engineering Congress & Exposition (IMECE 2011), 11-17 November 2011, Denver, Colorado.

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Abstract

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3239/8147
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	29 Nov 2011 15:51
Last Modified:	29 Nov 2011 15:51
URI:	http://irep.iium.edu.my/id/eprint/8147

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