Plackett-Burman design for the optimization of a novel extracellular marine protease production

Dehimi, Maya and Yusof, Faridah (2020) Plackett-Burman design for the optimization of a novel extracellular marine protease production. In: Asian Regional Conference on Systems Biology 2020, 2nd-4th March 2020, Langkawi, Kedah. (Unpublished)

	PDF (Poster) - Presentation Restricted to Repository staff only Download (1MB) | Request a copy
	PDF (Acceptance letter and schedule) - Supplemental Material Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Proteases are protein degrading enzymes, widely used in many enzyme-based industries. The search for novel proteases never stops. Proteases from marine organisms have several advantages over the proteases obtained from the non-marine environment since their optimal temperatures and other enzymatic characteristics differ significantly. In this study, marine bacteria were isolated from sea water and sediments from the South China Sea to be investigated on their ability to produce extracellular protease on skim milk agar plates and the production was confirmed biochemically by the protease assays. Three bacterial strains identified as Bacillus safensis, Bacillus licheniformis and Staphylococcus warneri were selected and the protease production were optimized by running experiments using the Plackett-Burman design. This approach facilitated the study of the effect of a large number of variables spanned by many factors and their settings with a small number of experimental runs leading to a considerable economy in time and cost for the process optimization. Bacillus licheniformis had shown to be the highest yielding protease, whereby the production levels increased from 43.76 U/mL to 158.41 U/mL. Factors observed to be significant were the concentrations of CaCl2, the inoculum size and agitation rate. This information is important in the next level of the optimization processes.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4734/79834
Uncontrolled Keywords:	Plackett-Burman design, novel extracellular, marine protease production
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	20 Apr 2020 14:49
Last Modified:	20 Apr 2020 14:49
URI:	http://irep.iium.edu.my/id/eprint/79843

Actions (login required)

View Item