Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis

Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Abdul Rahman, Russly and Abas, Faridah (2020) Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis. Journal of Analysis and Testing. pp. 1-12. ISSN 2096-241X E-ISSN 2509-4696

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Abstract

Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This study specifically compared the coating of capillary column, sample derivatization, and temperature programming of GC/MS to determine the composition of CPSE. The retention indices (RI) of the detected compounds were determined and compared to the reference RI. In particular, 5% phenyl–95% methylpolysiloxane (HP-5MS)-, DB-WAX-, and biscyanopropyl polysiloxane (HP-88)-coated capillary columns were used to identify the composition of CPSE. For this study, HP-5MS column, which separated the highest number of compounds (26 compounds) from CPSE, was deemed as the most suitable column. The GC/MS analysis of derivatized CPSE identified 21 compound groups, where fatty acids and fatty acid methyl esters served as the major compounds (80.23%), followed by these compounds in decreasing order: amides > nitriles > sterols > fatty aldehydes > organic acids. A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming (LTP) (R2 = 0.9859) was found, as compared to its correlation determination with NLTP (R2 = 0.9175), which exhibited an almost equal RI of LTP to the reference RI. Conclusively, GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended.

Item Type:	Article (Journal)
Additional Information:	7834/79437
Uncontrolled Keywords:	Capillary column, Carica papaya seed, GC/MS, Sample derivatization, Temperature programming
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Muhamad Shirwan Abdullah Sani
Date Deposited:	10 Mar 2020 09:15
Last Modified:	25 Nov 2020 15:36
URI:	http://irep.iium.edu.my/id/eprint/79437

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