Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis

AKMAN, AKMA AZMIERA and Talib, Ain Aisyah and Md Yusof, Azirah and Khalid, Kamarul Ariffin and Yusof, Nurul Asyiqin and Abdul Ghani, Radiah and Hamdan, Asmah Hanim and Zamli, Zaitunnatakhin (2018) Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis. Malaysian Applied Biology. ISSN 0126-8643

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Abstract

Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the subchondral bone plate plays an important role in the pathogenesis of osteoarthritis.

Item Type:	Article (Journal)
Additional Information:	5401/77748
Uncontrolled Keywords:	Osteoarthritis, osteoprotegerin, receptor activator of nuclear factor kb ligand, subchondral bone plate, trabecular bone
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation Kulliyyah of Medicine > Department of Pathology & Lab Medicine Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Zaitunnatakhin Zamli
Date Deposited:	28 Jan 2020 11:59
Last Modified:	28 Jan 2020 11:59
URI:	http://irep.iium.edu.my/id/eprint/77748

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