Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells

Md Hashim, Siti Nurnasihah and Yusof, Muhammad Fuad Hilmi and Zahari, Wafa’ and Chandra, Hamshawagini and Ahmad Amin Noordin, Khairul Bariah and Kannan, Thirumulu Ponnuraj and Sheikh Abdul Hamid, Suzina and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2019) Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells. Cellular and Molecular Bioengineering, Vol. 12 (6). pp. 599-613. ISSN 1865-5025 E-ISSN 1865-5033

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Abstract

Introduction—Endothelial cells cover the surface of the capillary wall and literature review has cemented its angiogenic roles in wound healing and tissue regeneration. However, the angiogenic in vitro models available are inadequate to understand the endothelial differentiation process. Methods—A construct was made using human amniotic membrane (HAM) as a matrix to assist the dental stem cells to differentiate into endothelial-like cells. This study aimed to assess the biological interaction between stem cells from human exfoliated deciduous teeth (SHED) and the stromal side (SS) of the glycerol-preserved HAM in angiogenic induced environment media using VEGF. The changes were evaluated through cell morphology, migration, as well as gene expression level. Results—There were morphological changes observed in SHED in angiogenic-induced media. SHED appeared to be differentiated from fibroblast-like cells to a new structure, mimicking endothelial-like structure through microscopy analysis. Besides, the cross-section of the construct revealed that the cells seeded on the matrix were able to maintain its monolayer structure at day 1, 7 and 14 but infiltrated into the HAM at day 21, suggesting cell migration. The cells were also able to maintain its stemness (Nestin, Nanog and CD29) and at the same time express the angiogenic markers (IL-8, VEGF and MMP-2). Conclusion—HAM promotes SHED proliferation, migration and has the potential as a differentiating matrix for endothelial-like cells.

Item Type:	Article (Journal)
Additional Information:	7172/75120
Uncontrolled Keywords:	angiogenic, endothelial differentiation, extracellular matrix, regenerative medicine, stem cells from human exfoliated deciduous teeth, vascular endothelial growth factor.
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	04 Dec 2019 17:47
Last Modified:	13 Apr 2020 13:17
URI:	http://irep.iium.edu.my/id/eprint/75120

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