Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds

Mahmud, Israa Ali and Mirghani, Mohamed Elwathig Saeed and Al-Qubaisi, Mothanna and , Beh Yee and Ismail, Norsharina and Rasedee, Abdullah and Yusof, Faridah and Shahabuddin, Munira and Al-khatib, Ma'an and Kabbashi, Nassereldeen Ahmed and Hashim, Yumi Zuhanis Has-Yun and Hilles, Ayah and Abbas, Phirdaous (2019) Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds. Preprint. pp. 1-37. (Unpublished)

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Abstract

Background and objectives: The initiation of apoptotic death in cancer cells is the aim of many chemotherapeutic strategies. As many anticancer agents have been discovered in natural plant sources, the search for novel phytochemicals displaying anticancer activities continues at a tremendous rate. This study was conducted to evaluate the effect of extractable polyphenols (EPP); non-extractable polyphenols (NEPP); nobiletin (NOB); tectorigenin (TEC) and persicogenin (PERS) from Barhi date palm kernels (BDPK) extracts on the induction of apoptosis in human lung cancer A549 and colon cancer HT29 cell lines. Materials and Methods: The anticancer activities were determined using MTT and trypan blue exclusion assays. Flow cytometry analysis was performed to study the cell viability, cell cycle arrest and apoptosis. The underlying mechanism of apoptosis induced by crude extracts and the purified compounds was investigated using caspase-3, -8 and -9 assays and the mitochondrial membrane potential assay. Results: The findings indicate that both BDPK extracts and the purified compounds do exert induced cell death on A549 and HT29 cells. The results observed from MTT- assay and trypan blue exclusion indicate that the cytotoxic effects of both plant extracts and the three isolated flavonoids are dose-dependent with higher cell death after 72 hours treatment. Treatment of human lung and colon cancer cells with EPP, NEPP, NOB, TEC and PERSI induced late stages of apoptosis as there was evidence of the DNA degradation and high percentage of the cells population was situated at sub-G1 phase, indicating a high population of apoptotic cells. Conclusions: Study of the apoptotic mechanism demonstrated that EPP and NEPP exhibited dependent mitochondrial signalling pathway as seen with caspase-9 and induced receptor-mediated (extrinsic) apoptotic pathway as seen with caspase-8. Therefore, our results suggest that BDPK extracts and the three isolated flavonoids could be a good candidate for developing anticancer agents.

Item Type:	Article (Electronic Media)
Additional Information:	4971/74977
Uncontrolled Keywords:	Apoptosis; Barhi date palm kernels (BDPK); extractable polyphenols (EPP); non-extractable polyphenols (NEPP); nobiletin (NOB); tectorigenin (TEC); persicogenin (PERSI)
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	A Prof Elwathig Mirghani
Date Deposited:	08 Nov 2019 15:05
Last Modified:	08 Nov 2019 15:06
URI:	http://irep.iium.edu.my/id/eprint/74977

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