Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain

Ismail, Nurul Azira and Amid, Azura (2011) Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

It is important to know the thermal and storage behavior of produced protein for the purpose of storage, transport and shelve life during industrial application. Differential Scanning Calorimetry (DSC) is used to measure the thermal and storage stability of recombinant bromelain produced in our laboratory. Recombinant bromelain thermal and storage stability were measured and compared to the commercial bromelain. The purified recombinant bromelain was powderized using freeze drying method and its transition midpoint, crystalline and enthalpy were recorded. Recombinant bromelain is more stable than commercial bromelain at higher temperature but the stability reduced after 7 days of storage at 4oC. Moreover, its activity is lower than commercial at day 1 and 7. Higher energy is needed to break the bond between amino acid chains in recombinant bromelain showing by the enthalpy recorded suggesting that recombinant bromelain has good protein structure and conformation compared to commercial.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3688/7470 (proceeding of the 2nd International Conference on Biotechnology Engineering (ICBioE 2011)) ISBN: 978-983-42978-3-
Uncontrolled Keywords:	DSC; thermal stability; storage stability; recombinant bromelain.
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	29 Nov 2011 22:20
Last Modified:	19 Jan 2012 11:40
URI:	http://irep.iium.edu.my/id/eprint/7470

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