Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia

Alaraidh, Ibrahim and Amid, Azura and Abd-El Aziem, Farouk and Ibrahim, Mohamed M. and Bazaid, Salih and Greiner, Ralf and Alghunaim, Abdullah (2011) Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia. IUFS Journal of Biology, 70 (1). pp. 11-17. ISSN 1300-7041

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Abstract

A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.

Item Type:	Article (Journal)
Additional Information:	3688/7440
Uncontrolled Keywords:	NOS terminator detection, genetically modified organism, processed foods, Rice, PCR
Subjects:	S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	29 Nov 2011 22:09
Last Modified:	25 Apr 2012 09:30
URI:	http://irep.iium.edu.my/id/eprint/7440

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