Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile

Jaswir, Irwandi and ,, Dedi Noviendri and Bakhtiar, Muhammad Taher and Mohamed, Farahidah and Octavianti, Fitri and Lestari, Widya and Mukti, Ali Ghufron and Nirwandar, Sapta and Almansori, Bubaker B. Hamad (2019) Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile. Molecules, 24 (5). pp. 1-16. ISSN 1420-3049

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Abstract

Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and optimize particle size (PS) and encapsulation efficiency (EE). Using response surface methodology (RSM), a face centered central composite design (FCCCD) was designed with three factors: Polyvinylalcohol (PVA), poly(D,L-lactic-co-glycolic acid) (PLGA), and fucoxanthin concentration. F-LM was produced using a modified double-emulsion solvent evaporation method. The F-LM were characterized for release profile, release kinetics, and degradation pattern. Optimal F-LM PS and EE of 9.18 µm and 33.09%, respectively, with good surface morphology, were achieved from a 0.5% (w/v) PVA, 6.0% (w/v) PLGA, 200 µg/mL fucoxanthin formulation at a homogenization speed of 20,500 rpm. PVA concentration was the most significant factor (p < 0.05) affecting PS. Meanwhile, EE was significantly affected by interaction between the three factors: PVA, PLGA, and fucoxanthin. In vitro release curve showed fucoxanthin had a high burst release (38.3%) at the first hour, followed by a sustained release stage reaching (79.1%) within 2 months. Release kinetics followed a diffusion pattern predominantly controlled by the Higuchi model. Biodegradability studies based on surface morphology changes on the surface of the F-LM, show that morphology changed within the first hour, and F-LM completely degraded within 2 months. RSM under FCCCD design improved the difference between the lowest and highest responses, with good correlation between observed and predicted values for PS and EE of F-LM.

Item Type:	Article (Journal)
Additional Information:	5784/71146
Uncontrolled Keywords:	fucoxanthin; release profile; response surface methodology; microsphere; microencapsulation
Subjects:	R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Prof. Dr. Irwandi Jaswir
Date Deposited:	19 Mar 2019 11:05
Last Modified:	01 Aug 2019 11:41
URI:	http://irep.iium.edu.my/id/eprint/71146

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