Optimization of serum preparation in Vero cell culture using a statistical approach

Ahmad Nor, Yusilawati and Nuhu Jaafar, Jaafar and Mel, Maizirwan (2009) Optimization of serum preparation in Vero cell culture using a statistical approach. In: 3rd International Conference on Chemical & Bioprocess Engineering , 12th–14th August 2009, Kota Kinabalu, Sabah. (Unpublished)

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Abstract

Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500ml whole blood from disease free cattle using 50ml anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30min at 56oC. Vero cells were cultured in 25cm2 T flasks with an initial cell seeding of 4.2 x 105 cells/ml. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 x 105 cells/ml) compared to commercial bovine serum (8.5x 105 cell/ml and 6.78x105 cell/ml) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4085/6300
Uncontrolled Keywords:	Local Process, Bovine Serum, Vero Cells, Growth Profile
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	24 Oct 2011 08:39
Last Modified:	14 Jan 2019 09:43
URI:	http://irep.iium.edu.my/id/eprint/6300

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