Evaluation of histological and biomechanical properties on engineered meniscus tissues using sonication decellularization

Mohamad Ghazali, Aina Mardhiyah and Sha'ban, Munirah and Noor Azmi, Azran Azhim (2017) Evaluation of histological and biomechanical properties on engineered meniscus tissues using sonication decellularization. In: 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 201), 11th-15th July 2017, Jeju Island; South Korea.

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Abstract

Sonication decellularization treatment requires proper evaluations on its ability to decellularize meniscus tissue efficiently. This study was done to evaluate the histological and biomechanical properties within meniscus scaffolds. Van Gieson staining was done to evaluate the efficiency of cell removal in meniscus tissues. The consequences of treatment on viscoelastic properties are vital for scaffolds quality and were properly investigated. Picrosirius red and Safranin-O/Fast green staining was carried out to detect extracellular matrix materials (ECM). Sonication decellularization treatment has the ability to demonstrated complete nuclei removal compare to control samples as well as maintaining viscoelastic properties, namely stiffness, compression and residual force. Thus, sonication decellularization treatment had successfully produced and prepared a meniscus bioscaffold candidate in which its biomechanical strength is sustained through protection of ECM properties.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	6006/61885
Uncontrolled Keywords:	Decellularization; sonication; menisci; scaffolds; biomechanical; extracellular matrix
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	12 Feb 2018 16:59
Last Modified:	26 Jun 2018 12:24
URI:	http://irep.iium.edu.my/id/eprint/61885

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