Optimization of process conditions for citric acid production utilizing STP sludge as substrate by liquid state bioconversion

Alam, Md. Zahangir and Jamal, Parveen and Saari, Rosnani (2006) Optimization of process conditions for citric acid production utilizing STP sludge as substrate by liquid state bioconversion. In: Regional Postgraduate Conference on Science and Engineering, 27-28 July, 2006, UTM, Malaysia.

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Abstract

Citric acid was produced by Aspergillus niger A-SS106 isolated from local sources utilizing sewage treatment plant (STP) sludge as substrate in laboratory scale process. Central composite design (CCD) was carried out to identify the total experiments for optimization of process conditions for citric acid production. The variables involved in the study were temperature, agitation rate, initial pH and inoculum size. A polynomial model was developed using the experimental data for determining the optimum process conditions through response surface methodology with respect to citric acid production. Statistical analysis of results showed the optimized values were: temperature of 30 ºC, agitation rate of 350 rpm, initial pH of 4 and inoculum size of 2%. Under the optimum conditions obtained from model equation, the predicted citric acid was 1.51 g/l produced on day 4. The maximum citric acid was 2.9 g/l obtained on day 2 under the optimum conditions.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4157/5846
Uncontrolled Keywords:	Citric acid, Liquid state bioconversion, Sewage treatment plant sludge, Process optimization
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	17 Nov 2011 11:38
Last Modified:	22 Nov 2011 06:46
URI:	http://irep.iium.edu.my/id/eprint/5846

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