The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study

Ali, Wathiq Q.S. and Selman, Mohammad Oda and Al-Hasson, Indaf J. and Al-Ani, Imad Matloub Dally and Al-Salihi, Anam R. (2017) The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study. Iraqi Journal of Medical Sciences, 15 (2). pp. 116-128. ISSN 0681-6579 E-ISSN 2224-4719

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Abstract

Dexamethasone is a glucocorticoid as a member of the steroidal anti-inflammatory and immunosuppressant. It has well documented effects on skeletal structures osseous and cartilaginous, commonly used to treat or control diseases. Objective To evaluate by histomorphometric study the effects of dexamethasone on the embryogenesis of long bones in chick embryos. Methods Forty-eight fertile chick eggs of Gallus gallus domesticus, were used. The eggs were divided into 2 groups; control and treated groups of 24 eggs each, these groups were subdivided into 4 subgroups (n=6 eggs). On day 10 of incubation, the control group was injected with 25 μl of distilled water while the treated group was injected with 25 μl of distilled water contained 8 μg dexamethasone. In the next days (11, 12, 13, and 14 of incubation), 12 chick embryos were sacrificed in each day. A computer-assisted morphometric/ image analysis (Motic Image Plus version 2.0ML), was used to measure length, area, perimeter of tibiae, and the area and perimeter of the perichondral osseous collar of cross section in mid-diaphyseal zone of these bones. Results These bones of chick embryos treated with dexamethasone, suffered shortening and retardation in length, weight, area and perimeter throughout the period of this study, decline area and perimeter to the perichondral osseous collar in the mid-diaphyseal zone. Conclusion Dexamethasone given at day 10 of incubation caused tibial bones growth retardation at development stages 11, 12, 13, and 14-days; this was observed in the measured parameters: bone length, area, perimeter and weight.

Item Type:	Article (Journal)
Additional Information:	5849/58341
Uncontrolled Keywords:	Bones, chick-embryo, dexamethasone, histomorphometry
Subjects:	Q Science > QM Human anatomy Q Science > QP Physiology R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Assoc. Prof Dr.. Imad Matloub Dally Al-Ani
Date Deposited:	14 Sep 2017 09:17
Last Modified:	01 Nov 2017 15:33
URI:	http://irep.iium.edu.my/id/eprint/58341

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