In vitro antioxidant, antityrosinase, antibacterial and cytotoxicity activities of the leaf and stem essential oil from piper magnibaccum C. DC.

Hashim, Nur Athirah and Ahmad, Farediah and Jani, Nor Akmalazura and Darnis, Deny Susanti (2017) In vitro antioxidant, antityrosinase, antibacterial and cytotoxicity activities of the leaf and stem essential oil from piper magnibaccum C. DC. Journal of Essential Oil Bearing Plants, 20 (1). pp. 223-232. ISSN 0972-060X E-ISSN 0976-5026

	PDF - Published Version Restricted to Repository staff only Download (493kB) | Request a copy
	PDF (SCOPUS) Restricted to Repository staff only Download (96kB) | Request a copy
	PDF (WOS) Restricted to Repository staff only Download (173kB) | Request a copy

Abstract

The chemical composition of essential oils obtained from two parts of Piper magnibaccum C.DC. were analyzed by gas chromatography with a flame ionization detector (GC- FID) and gas chromatography- mass spectrometry (GC- MS). 25 components representing 93.5 % of the essential oil were characterized from the leaf of P. magnibaccum. The essential oil was dominated by germacrene D (40.8 %), β-caryophyllene (8.5 %) and α-cadinol (6.1 %). A total of 33 components, representing 87.6 % of the total oil were identified from the stem of P. magnibaccum. The major constituents were β-caryophyllene (19.7 %), germacrene D (10.7 %), and α- cadinol (8.2 %). The antioxidant potential of the leaf and stem essential oils of P. magnibaccum were determined on the basis of phenolic content and scavenging activity of the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 20.5, 17.5 μg/mL) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) (IC50 11.7, 12.9 μg/ mL) free radicals. Antityrosinase activity of the leaf and stem were also screened using mushroom tyrosinase assay (I %= 49.50±0.6, 57.01±0.8). The essential oil of P. magnibaccum leaf showed a moderate antibacterial activity with MIC value of 250 μg/mL against P. aeruginosa. None of the essential oils exhibited acute cytotoxicity against MCF-7 and A-549 cells proliferation.

Item Type:	Article (Journal)
Additional Information:	5641/56457
Uncontrolled Keywords:	Essential oil, Piper magnibaccum, Piperaceae, Antioxidant, Antityrosinase, Antibacteria, Cytotoxic
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	27 Apr 2017 14:59
Last Modified:	18 Jul 2017 09:02
URI:	http://irep.iium.edu.my/id/eprint/56457

Actions (login required)

View Item