Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains

Alam, Md. Zahangir and Drani, Afifah and Muyibi, Suleyman Aremu (2010) Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains. In: International Conference on Environmental Science and Technology (ICEST 2010) , 23-25 April 2010, Bangkok, Thailand. (Unpublished)

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Abstract

A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4157/5583
Uncontrolled Keywords:	Domestic sewage sludge, Endo-β-1,4 glucanase activity, Trichoderma reesei
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	03 Nov 2011 16:01
Last Modified:	17 Jan 2012 12:05
URI:	http://irep.iium.edu.my/id/eprint/5583

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