Mat Nawi, Nur Farhana and Sha'ban, Munirah and Zainuddin, Zainul Ibrahim
(2016)
Effects of ionizing radiation on chondrocytes growth: work in progress.
In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17 - 18 Nov 2016, Penang.
(Unpublished)
Abstract
Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes.
Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches
80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a
typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage.
Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control.
Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes.
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