Co-production of lactic acid and ethanol using rhizopus sp. From hydrolyzed inedible cassava starch and leaves

Azmi, Azlin Suhaida and Yusuf, Nurlisa and Jimat, Dzun Noraini and Mohamad Puad, Noor Illi (2016) Co-production of lactic acid and ethanol using rhizopus sp. From hydrolyzed inedible cassava starch and leaves. IIUM Engineering Journal, 17 (2). pp. 1-10. ISSN 1511-788X E-ISSN 2289-7860

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Abstract

Production of lactic acid and ethanol from inedible cassava starch and leaves was investigated. Prior to fermentation, hydrolysis of the starch and leaves was conducted. Hydrolysis was optimized by manipulating at three levels each four particular factors, namely acid concentration, starch concentration, temperature, and reaction time. Maximum glucose yield of 0.96 g/g was obtained when 2.5% (w/v) of cassava leaves with 2.5% (w/v) of starch was hydrolyzed using 0.20 M nitric acid at 160 oC of temperature for 10 min. The potential of hydrolyzed cassava starch and leaves was then investigated for lactic acid production using fungal Rhizopus sp. The fermentation process was then conducted in a shake flask by varying four factors at three levels each. Maximum lactic acid and ethanol yields of 0.95 g/g and 0.52 g/g, respectively, were achieved at different optimum conditions. Lactic acid production was found to be linked to a decrease in ethanol production. Design Expert v6.0.8 was used to aid in the design of the experiment using Taguchi’s methodology.

Item Type:	Article (Journal)
Additional Information:	5339/53605
Uncontrolled Keywords:	cassava leaves; cassava starch; ethanol; lactic acid; Rhizopus sp.
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Mrs. Azlin Suhaida Azmi
Date Deposited:	29 Dec 2016 10:55
Last Modified:	10 Jan 2017 14:12
URI:	http://irep.iium.edu.my/id/eprint/53605

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