Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures

Ten, Seng Teik and Hashim, Uda and Gopinath, Subash C.B and Liu, W.W. and Foo, K.L. and Sam, S.T and Ab Rahman, Siti Fatimah and Voon, Chun Hong and Nordin, Anis Nurashikin (2017) Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures. Biosensors and Bioelectronics, 93. pp. 146-154. ISSN 0956-5663 E-ISSN 1873-4235

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Abstract

Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1 cfu E.coli O157:H7 in 25 g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130 nm thin layer of SiO2 nanostructures with particle size lesser than 70 nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide tar- get and attained the sensitivity of 0.6439 nM/0.1 kHz and detection limit was down to 1.8 femto-molar (1.8×10−15 M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.

Item Type:	Article (Journal)
Additional Information:	3239/52752
Uncontrolled Keywords:	E. coli O157:H7 Shear horizontal surface acoustic wave (SHSAW) Surface unctionalization, deoxyribonucleic acid (DNA) hybridization
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	08 Nov 2016 10:51
Last Modified:	16 May 2018 16:42
URI:	http://irep.iium.edu.my/id/eprint/52752

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