Fluorescence multispectral imaging-based diagnostic system for atherosclerosis

Ho, Cassandra Su Lyn and Horiuchi, Toshikatsu and Taniguchi, Hiroak and Umetsu, Araya and Hagisawa, Kohsuke and Iwaya, Keiichi and Nakai, Kanji and Azmi, Amalina and Zulaziz, Natasha and Azhim, Azran and Shinomiya, Nariyoshi and Morimoto, Yuji (2016) Fluorescence multispectral imaging-based diagnostic system for atherosclerosis. BioMedical Engineering OnLine, 15 (98). pp. 1-10. ISSN 1475-925X

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Abstract

Background Composition of atherosclerotic arterial walls is rich in lipids such as cholesterol, unlike normal arterial walls. In this study, we aimed to utilize this difference to diagnose atherosclerosis via multispectral fluorescence imaging, which allows for identification of fluorescence originating from the substance in the arterial wall. Methods The inner surface of extracted arteries (rabbit abdominal aorta, human coronary artery) was illuminated by 405 nm excitation light and multispectral fluorescence images were obtained. Pathological examination of human coronary artery samples were carried out and thickness of arteries were calculated by measuring combined media and intima thickness. Results The fluorescence spectra in atherosclerotic sites were different from those in normal sites. Multiple regions of interest (ROI) were selected within each sample and a ratio between two fluorescence intensity differences (where each intensity difference is calculated between an identifier wavelength and a base wavelength) from each ROI was determined, allowing for discrimination of atherosclerotic sites. Fluorescence intensity and thickness of artery were found to be significantly correlated. Conclusions These results indicate that multispectral fluorescence imaging provides qualitative and quantitative evaluations of atherosclerosis and is therefore a viable method of diagnosing the disease.

Item Type:	Article (Journal)
Additional Information:	7380/52526
Uncontrolled Keywords:	Atherosclerosis, Multispectral fluorescence imaging, Abdominal aorta, Coronary artery
Subjects:	R Medicine > RB Pathology R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	04 Nov 2016 12:26
Last Modified:	21 Dec 2016 15:43
URI:	http://irep.iium.edu.my/id/eprint/52526

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