A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components

Amid, Mehrnoush and Asmadi, Farhana Azmira and Hussina, Muhaini and Manap, Mohd Yazid and Sarker, Md. Zaidul Islam and Hean, Chong Gun (2016) A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components. Separation Science and Technology, 51 (6). pp. 968-975. ISSN 0149-6395 E-ISSN 1520-5754

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Abstract

A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%.

Item Type:	Article (Journal)
Additional Information:	6605/51508
Uncontrolled Keywords:	Novel aqueous micellar two phase system; Surfactant; Mannitol; Recycling of phase components; Polygalacturonase; Durio zibethinus Murry
Subjects:	Q Science > Q Science (General) T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Professor Md. Zaidul Islam Sarker
Date Deposited:	13 Feb 2018 11:25
Last Modified:	21 Jun 2018 10:47
URI:	http://irep.iium.edu.my/id/eprint/51508

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