Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration

Mohamad Sukri, Norhamiza and Razali, Rabiatul Adawiyah and Arifin, Nurfadzillah and Harun, Nurul Athirah and Mohd Azid @ Nazid, Ruwaida and Suboh, Ruzira and Mohamad Sofian, Nurul Damia and Abdul Rahman, Suzanah and Abdul Rahim, Muhd Afzam Shah and Sha'ban, Munirah (2015) Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration. Regenerative Research, 4 (1). pp. 13-22. E-ISSN 2232-0822

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Abstract

Devoid of scientific proofs, the use of commercially available natural product-based eye toners for therapeutic purposes incites controversies surrounding it. This study aims to evaluate the effect of selected commercially available eye toners on in vitro cellular model using rabbits’ corneal keratocytes. The primary cells were cultivated in culture media supplemented with or without eye toners as control. Growth kinetic profile, cells proliferation assay, scratch assay and immunocytochemistry analysis were performed in comparing both culture media. There were no significant differences between control and the commercially available eye toners in the growth kinetics and cell proliferation. Microscopic observation of the scratch assay suggested the eye toners did not promote cells migration as compare to control. However, in the immunohistochemistry study the eye toners are able to sustain the expression of Connexin-43 and Lumican. This preliminary study indicates that the selected eye toners do not promote the proliferation and migration of corneal keratocytes. They are able to maintain the expression of Connexin-43 and Lumican which might have beneficial effect in corneal transparency. Studies with individual element of the eye toners need to be done.

Item Type:	Article (Journal)
Additional Information:	6006/47518 Corresponding Author: Dr. Munirah Sha’ban
Uncontrolled Keywords:	Eye toners, Cornea, Keratocytes, Cellular model
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Optometry and Visual Science
Depositing User:	Munirah Sha'ban
Date Deposited:	06 Jan 2016 20:46
Last Modified:	15 Jul 2016 10:53
URI:	http://irep.iium.edu.my/id/eprint/47518

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