Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production

Ibrahim Ali , Noorbatcha and Hamzah, Mohd. Salleh (2015) Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production. In: ASIAN Congress on Biotechnology 2015, 15th-19th Nov. 2015, Kuala Lumpur.

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Abstract

Effective bioconversion of lignocellulosic biomass into soluble sugars for bioethanol production requires use of thermostable and highly active cellulases. Lignocellulose is the most abundant biopolymer on earth and it is a good renewable energy source. Thermostable enzymes have several benefits in various industrial applications. This research intends to predict computationally, and develop experimentally, a thermostable mutant endoglucanase that can operate at higher temperature as required by industrial processes. Our preliminary computational data based on rational design approach suggest that a quintuple mutant (T224E/G229A/S230F/S231E/N321R) of an endoglucanase from a pathogenic fungus, Fusarium oxysporum, can potentially remain active at higher temperature (80oC) compared to its current operating temperature (<60oC). In our effort to experimentally produce this quintuple mutant we have successfully produced few mutants via site directed mutagenesis. We are reporting here the characterization results of these mutants as compared to the wild type. Keywords: Thermostable enzymes, mutant endoglucanases, Fusarium oxysporum

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	3704/46336
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited:	06 Jan 2016 17:17
Last Modified:	24 May 2016 08:06
URI:	http://irep.iium.edu.my/id/eprint/46336

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