Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice

Abdul Ghani, Radiah and Md Yusof, Najwa Farhah and Nik Abdul Malek, Nik Nurasyikin and A.Talib, Norlelawati (2015) Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10-12 August 2015, Impiana Resort Cherating, Pahang, Malaysia. (Unpublished)

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Abstract

The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4458/44455
Uncontrolled Keywords:	polyamines, Ornithine decarboxylase (ODC), A549, chemoprevention, natural product
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr. Radiah Abdul Ghani
Date Deposited:	07 Sep 2015 15:37
Last Modified:	03 Jan 2018 09:26
URI:	http://irep.iium.edu.my/id/eprint/44455

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