Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract

Sulaiman, Fatimatul Akmal and Fuad, Nurfarahain and Rahman, Farawahida and Iqbal, Anwar and Darnis, Deny Susanti (2015) Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Cherating, Pahang. (Unpublished)

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Abstract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the methanolic extract were investigated by using TPC, TFC, DPPH Free Radical Scavenging Assay and β –carotene bleaching assay. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the methanolic extract and ascorbic acid were 0.21 and 0.04 mg/mL respectively. The average percentage of β–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	5641/44397
Uncontrolled Keywords:	Tinospora crispa stems methanolic extract
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	05 May 2016 08:47
Last Modified:	22 May 2018 11:50
URI:	http://irep.iium.edu.my/id/eprint/44397

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