A novel animal model for subcutaneous soft tissue infection

Zulaziz, Natasha and N. , Himeno and H. , Miyazaki and D. , Saitoh and Noor Azmi, Azran Azhim and Y. , Morimoto (2014) A novel animal model for subcutaneous soft tissue infection. Biomedical Engineering, 52. pp. 285-286. ISSN 1881-4379

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Abstract

In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence. Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.

Item Type:	Article (Journal)
Additional Information:	7380/44152
Uncontrolled Keywords:	MRSA, neutrophil, infection
Subjects:	R Medicine > RZ Other systems of medicine T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	18 Aug 2015 08:20
Last Modified:	18 Aug 2015 08:20
URI:	http://irep.iium.edu.my/id/eprint/44152

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