The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds

Azhim, A and Syazwani, N and Morimoto, Y and Furukawa, Ks and Ushida, T (2013) The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds. Journal of biomaterials applications, 29 (1). pp. 130-141. ISSN 1530-8022

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Abstract

A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.

Item Type:	Article (Journal)
Additional Information:	7380/43658
Uncontrolled Keywords:	Decellularization, extracellular matrices, sonication, bioscaffolds, aortic tissue, implantation
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	14 Jul 2015 11:19
Last Modified:	14 Jul 2015 11:19
URI:	http://irep.iium.edu.my/id/eprint/43658

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