Cytotoxicity and inhibition of nitric oxide in lipopolysaccharide induced mammalian cell lines by aqueous extracts of brown seaweed

Jaswir, Irwandi and Monsur, Hammed Ademola and Simsek, Senay and Amid, Azura and Alam, Md Zahangir and Salleh, Mohammad Noor and Tawakalit, Asiyanbi-Hammed and Octavianti, Fitri (2014) Cytotoxicity and inhibition of nitric oxide in lipopolysaccharide induced mammalian cell lines by aqueous extracts of brown seaweed. Journal of Oleo Science, 63 (8). pp. 787-794. ISSN 1345-8957 E-ISSN 1347-3352

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Abstract

Aqueous extracts obtained from five Malaysian brown seaweeds; Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata, were investigated for their abilities to inhibit Nitric oxide (NO) production in lipopolysaccharide (LPS) induced macrophage RAW 264.7 cell lines as well as their chemical composition. Percentage yield of extracts varied among species, with the least yield in P. australis and highest yield in T. turbinata. The chemical compositions of the extracts showed that percentage sulfate ion, uronic acid and total sugar contents vary remarkably. All extracts contain high fucose and inhibit NO secretion in a dose dependent manner. Percentage of NO inhibition correlates very well with uronic acid content of the extract. Extracts of P. australis and T. turbinata dosed as 200 g/ml where able to cause >75% NO inhibition. Cytotoxicity assay revealed that some extracts were moderately toxic while some are not. Brown seaweed of Malaysian origin can be further explored for additional anti-inflammatory biocompounds.

Item Type:	Article (Journal)
Additional Information:	5784/39694
Uncontrolled Keywords:	anti-inflammatory, macro-algae, polysaccharide, fucoidan, cytotoxicity
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Irwandi Jaswir
Date Deposited:	15 Dec 2014 14:44
Last Modified:	03 Aug 2017 15:24
URI:	http://irep.iium.edu.my/id/eprint/39694

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