Kinetics of lipase production from candida cylindracea by solid-state bioconversion of palm kernel cake

Alam, Md Zahangir and Elgharbawy, Amal Ahmed and Mohd Salleh, Hamzah (2014) Kinetics of lipase production from candida cylindracea by solid-state bioconversion of palm kernel cake. Asian Journal of Microbiology, Biotechnology & Environmental Sciences, 16 (3). pp. 523-528. ISSN 0972-3005

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Abstract

Solid- state bioconversion (SSB) of palm kernel cake (PKC) by Candida cylindracea is a cost-effective method to produce lipase from abundant raw material in Malaysia. This study was conducted to describe the growth profile of Candida cylindracea on PKC using SSB. Since separating yeast cells (biomass) is not a feasible task, indirect method was used to monitor the growth during the fermentation process. This method was based on glucosamine, protein and carbohydrate content of the fermented media during the fermentation period. The results showed that the pH of the fermented PKC increased from 6.1 to 8.5 at the first 60 h and turned to neutral after that. Protein concentration of the fermented medium started to decrease within the first 24 h, and then increased gradually. Total carbohydrates decreased from 290 mg/g PKC to reach 155 mg/g PKC by the end of the fermentation. Glucosamine, a component in the mycelia cell walls, was measured to monitor the biomass during the fermentation. Maximum concentration of glucosamine achieved after 72 h and started to decrease to almost stable for the next hours. This data can be used to have a good understanding of the fermentation process and the lipase production.

Item Type:	Article (Journal)
Additional Information:	4157/39131
Uncontrolled Keywords:	Lipase, Candida cylindracea, Palm kernel cake, Growth, Productivity, Protein
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	13 Nov 2014 11:47
Last Modified:	06 Sep 2017 11:47
URI:	http://irep.iium.edu.my/id/eprint/39131

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