Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells

Siddiquei, H. R. and Nordin, Anis Nurashikin and Ibrahimy, Muhammad Ibn and Arifin, Mohd Azmir and Sulong, Nurul Hafizah and Mel, Maizirwan and Voiculescu, Ioana (2010) Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells. In: International Conference on Computer and Communication Engineering (ICCCE 2010), 11-13 May 2010, Kuala Lumpur.

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Abstract

Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3239/3733
Uncontrolled Keywords:	Au;DF-1 cells;Teflon cell holder;biosensor;capacitance values;capacitors;cell attachment;cell culture media;cell growth;cell mobility;chicken embroyonic fibroblasts;electrical cell-substrate impedance sensing;equivalent circuit;gold electrodes;real time monitoring;resistance values;resistors;time 6 h;bioelectric phenomena;biological techniques;biosensors;capacitance measurement;cellular biophysics;electric impedance measurement;electric resistance measurement;electrodes;gold;zoology;
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices > TK7885 Computer engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	22 Sep 2011 08:45
Last Modified:	13 Nov 2011 16:23
URI:	http://irep.iium.edu.my/id/eprint/3733

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