Characterization of endothelial cells using electrochemical impedance spectroscopy

Liu, Fei and Arifuzzaman, S. M. and Nordin, Anis Nurashikin and Spray, David and Voiculescu, Ioana (2010) Characterization of endothelial cells using electrochemical impedance spectroscopy. In: Asia Pacific Conference on Circuits and Systems (APCCAS 2010), 6-9 Dec 2010, Kuala Lumpur.

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Abstract

In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cell layer in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements of endothelia cells were also simulated using COMSOL Multiphysics #x2122;, a commercially available modeling package.

Item Type:	Conference or Workshop Item (Lecture)
Additional Information:	3239/3702
Uncontrolled Keywords:	COMSOL multiphysics;ECIS-8W1E;eight-well cell culture impedance array;electrical cell-substrate impedance sensing;electrochemical impedance spectroscopy;endothelial cell lines;equivalent circuit model;frequency 100 Hz to 100 kHz;impedance measurement;biochemistry;biological specimen preparation;cellular biophysics;electrochemical impedance spectroscopy;equivalent circuits;
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices > TK7885 Computer engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	22 Sep 2011 11:18
Last Modified:	22 Sep 2011 11:18
URI:	http://irep.iium.edu.my/id/eprint/3702

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