Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion

Elgharbawy, Amal A.M. and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2013) Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 July 2013, Kuala Lumpur, Malaysia.

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Abstract

Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The factors that contributed the most in lipase production were further investigated using one-factor-at- a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was used for optimization afterword.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Additional Information:	4157/34999 (ISBN: 978-983-42978-6-2)
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	28 Jan 2014 11:00
Last Modified:	29 Jun 2021 10:59
URI:	http://irep.iium.edu.my/id/eprint/34999

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